Journal: Molecules
Article Title: Aspects of a Distinct Cytotoxicity of Selenium Salts and Organic Selenides in Living Cells with Possible Implications for Drug Design
doi: 10.3390/molecules200813894
Figure Lengend Snippet: Figure 2. Generation of intracellular ROS by Na2SeO3 in different strains of S. aureus. ATCC 25923 (a); HEMSA 5 (b) and HEMSA 5M (c). Data is shown in terms of fluorescence emitted (in arbitrary units) by (oxidized) DCFA. The latter is generated by the reaction of certain ROS and Reactive Nitrogen Species (RNS) with DCHFA. Different concentrations of Na2SeO3 were assayed and the influence of the dual presence of the chalcogen salt and an antibiotic (oxacillin) on ROS generation was also evaluated. Values shown represent mean values with n = 4. The error bars represent the standard deviation (SD) and statistical significances were calculated using an one-way ANOVA followed by Bonferroni’s multiple comparison test (Figure 2a,b: p > 0.05; 2c: p < 0.05).
Article Snippet: Bacterial Strains Wild-type S. aureus American Type Culture Collection ATCC 25923 (oxacillin MIC 0.2 μg/mL) and clinical methicillin resistant MRSA strains HEMSA 5 (oxacillin MIC 150 μg/mL) and MRSA HEMSA 5M (oxacillin MIC 2400 μg/mL) were used as part of this study (Instituto de Higiene e Medicina Tropical, Universidade Nova, Lisbon, Portugal).
Techniques: Fluorescence, Generated, Standard Deviation, Comparison